Dr. Andrew Joseph Kouba

Wang, M., Wang, G., Huang, G., Kouba, A. J., Swaisgood, R. R., Zhou, W., Hu, Y., Nie, Y., Wei, F. 2024. Habitat connectivity drives panda recovery. Current Biology

Saylor, E. M., Kouba, A. J., Boudreau, M. R., Songassen, N., Vance, C. 2024. Efficacy of Salmon GnRH, Ovaprim®, and hCG for Hormonal Stimulation of Spermiation in Amphibians. Conservation Physiology 12(1):coae056.

Chen, D. m., Chen, L., Vance, C. K., Songsasen, N., Roth, T. L., Kouba, A. J. 2024. Oral administration of GnRH via a cricket vehicle stimulates spermiation in tiger salamanders (Ambystoma tigrinum). . Plos One 19(7):1-15.

Sheng, Q., Santos-Rivera, M., Kouba, A. J., Wang, G., Vance, C. K. 2024. Near infrared spectroscopy as a field-based methodology for sex identification from feces of the Giant Panda . Conservation Biology

Chen, L., Caprio, M. A., Chen, D. M., Kouba, A. J., Vance, C. K. 2024. Enhancing predictive performance for spectroscopic studies in wildlife science through a multi-model approach: A case study for species classification of live amphibians. Plos Computational Biology 20(2):24.

Chen, D. M., Vance, C. K., Songsasen, N., Roth, T. L., Allen, P. J., Kouba, A. J. 2024. Comparing novel sperm extenders for the internally-fertilizing tiger salamander (Ambystoma tigrinum). . Frontiers in Amphibian and Reptile Science 1(e1320803):12.

Marcec-Greaves, R., Vance, C., Willard, S. T., Kouba, A. J. 2023. Ovarian ultrasound analysis for developing temporal and spatially explicit hormone regimens for induced ovulation and egg deposition in the tiger salamander. Theriogenology Wild

Julien, A. R., Counsell, K. R., Burger, I. J., Kouba, A. J., Barber, D., Cobos, C., Jennings, R. D., Christman, B. L., Vance, C. K. 2023. Effects of wild, semi-captive, and captive management on male Chiricahua leopard frog sperm quality withimplications for conservation breeding programs. Conservation Science and Practice 5(5):1-10.

Burger, I. J., Chen, L., Lampert, S. S., Vance, C., Barber, D., Cobos, C., Kouba, A. J. 2023. Applying sperm collection and cryopreservation protocols developed in a model amphibian to three threatened anuran species targeted for biobanking management. Biological Conservation 277(109850).

Lampert, S. S., Burger, I. J., Julien, A. R., Gillis, A. B., Kouba, A. J., Barber, D., Vance, C. K. 2022. Sperm Cryopreservation as a Tool for Amphibian Conservation: Production of F2 Generation Offspring from Cryo-Produced F1 Progeny. Animals 13(53):1-10.

Burger, I., Lampert, S., Vance, C. K., Morin, D., Kouba, A. J. 2022. Development of an amphibian sperm biobanking model for genetic management and population sustainability. Conservation Physiology 10(1):1-16.

Chen, D. M., Moore, M., Willis, E., Kouba, A. J., Vance, C. K. 2022. The impact of time and environmental factors on the mitochondrial vesicle and subsequent motility of amphibian sperm. Comparative Biochemistry and Physiology, Part A: Molecular & Integrative Physiology 268(111119):1-8.

Sheng, Q., Santos-Rivera, M., Ouyang, X., Kouba, A. J., Vance, C. K. 2022. Near-Infrared Spectroscopy and Mode Cloning (NIR-MC) for in-situ analysis of crude protein in bamboo. Remote Sensing 14(6):1302.

Chen, L., Santos-Rivera, M., Burger, I. J., Kouba, A., Barber, D., Vance, C. 2021. Near-Infrared Spectroscopy (NIRS) as a Method for Biological Sex Discrimination in the Endangered Houston Toad (Anaxyrus houstonensis). Methods and Protocols 5(1):4-16.

Burger, I., Julien, A. R., Kouba, A., Counsell, K. R., Barber, D., Vance, C. 2021. Linking in-situ and ex-situ populations of the endangered Puerto Rican crested toad (Peltophryne lemur) through biobanking of hormonally induced sperm. Conservation Science and Practice 3(11):e525.

Arrugei, L., Kouba, A., Germano, J., Barrios, L., Moore, M., Vance, C. 2021. Fertilization potential of cold-stored Fowler’s toad (Anaxyrus fowleri) spermatozoa: temporal changes in sperm motility based on temperature and osmolality. Reproduction, Fertility and Development 34(5):461-469.

Kouba, A., Langhorne, C. J., Willard, S., Smith, T., Vance, C. 2021. Spermiation response to exogenous hormone therapy in hibernated and non-hibernated boreal toads (Anaxyrus boreas boreas). Reproduction Fertility and Development. Reproduction, Fertility and Development 34(5):453-460.

Langhorne, C. J., Calatayud, N., Vance, C. K., Willard, S. T., Smith, T., Ryan, P. L., Kouba, A. J. 2021. Efficacy of hormone stimulation on sperm production in an alpine amphibian (Anaxyrus boreas boreas) and the impact of short-term storage on sperm quality. Zoology 146:125912.

Bronson, E., Guy, E., Murphy, K., Barrett, K., Kouba, A., Poole, V., Vance, C. 2021. Influence of oviposition-inducing hormone on spawning and mortality in the endangered Panamanian golden frog (Atelopus zeteki). BMC Zoology 6(17):1-14.

Wang, Q., Zhang, L., Zhao, H., Zhao, Q., Deng, J., Kong, F., Jiang, W., Zhang, H., Liu, H., Kouba, A. J. 2021. Abiotic and Biotic Influences on the Movement of Reintroduced Chinese Giant Salamanders (Andrias davidianus) in Two Montane Rivers. Animals 11:1480.

Gillis, A. B., Guy, E. L., Kouba, A. J., Allen, P. J., Marcec-Greaves, R. M., Vance, C. 2021. Short-term storage of tiger salamander (Ambystoma tigrinum) spermatozoa: The effect of collection type, temperature and time. PLOS ONE 16(1):e0245047.

Arregui, L., Boveda, P., Gosalvez, J., Kouba, A. J. 2020. Effect of seasonality on amphibian sperm production, storage and cryopreservation. Aquaculture 159(735677).

Guy, E. L., Gillis, A. B., Kouba, A. J., Barber, D., Poole, V., Marcec-Greaves, R., Vance, C. 2020. Sperm Collection and Cryopreservation for Threatened Newt Species. Cryobiology 94:80-88.

Guy, E. L., Martin, M. W., Kouba, A. J., Cole, J. A., Vance, C. 2020. Evaluation of different temporal periods between hormone-induced ovulation attempts in the female Fowler’s toad Anaxyrus fowleri. Conservation Physiology 8(1):8.

Arregui, L., Diaz-Diaz, S., Alonso-López, E., Kouba, A. J. 2019. Hormonal induction of spermiation in a Eurasian bufonid (Epidalea calamita). Reproductive Biology and Endocrinology 17(1):92.

Zhang, L., Zhao, H., Willard, S. T., Wang, Q., Jiang, W., Zhang, H., Kouba, A. J. 2019. Spatial distribution and seasonal movement patterns of reintroduced Chinese giant salamanders. BMC Zoology 4(7).

Julien, A. R., Kouba, A. J., Kabelic, D., Nguekam Feugang, J., Willard, S. T., Vance, C. K. 2019. Nasal administration of gonadotropin releasing hormone (GnRH) elicits sperm production in Fowler’s toads (Anaxyrus fowleri). BMC Zoology 4(3).

Roberts, B. M., Brown, J. L., Kersey, D. C., Snyder, R. J., Durrant, B. S., Kouba, A. J. 2018. Use of urinary 13,14, dihydro-15-keto-prostaglandin F_2α (PGFM) concentrations to diagnose pregnancy and predict parturition in the giant panda (Ailuropoda melanolecua). PloS One 13(5):e0195599.

Graham, K. M., Langhorne, C. J., Vance, C., Willard, S. T., Kouba, A. J. 2018. Ultrasound imaging improves hormone therapy strategies for induction of ovulation and in vitro fertilization in the endangered dusky gopher frog (Lithobates sevosa). Conservation Physiology 6(1):coy020.

Wilson, A. E., Sparks, D. L., Knott, K., Willard, S. T., Kouba, A. J., Brown Johnson, A. E. 2018. Behavioral, Semiochemical and Androgen Responses by Male Giant Pandas to the Olfactory Sexual Receptivity Cues of Females. Theriogenology 114:330-337.

Williams, C. L., Dill-McFarland, K., Sparks, D. L., Kouba, A. J., Willard, S. T., Suen, G., Brown, A. E. 2018. Dietary changes during weaning shape the gut microbiota of red pandas (Ailurus fulgens). Conservation Physiology 6(1).

Gocinski, B. L., Knott, K. K., Roberts, B. M., Brown, J. L., Vance, C., Kouba, A. J. 2018. Changes in urinary androgen concentration indicate that male giant pandas (Ailuropoda melanoleuca) respond to impending female oestrus during and outside the typical spring breeding season. Reproduction, Fertility, and Development 30(2):399-408.

Gocinski, B., Knott, K., Roberts, B., Brown, J., Vance, C., Kouba, A. J. 2017. Changes in urinary androgen concentration indicate that male giant pandas (Ailuropoda melanolueca) respond to impending female estrus during and outside of typical spring breeding season. Reproduction, Fertility and Development 30(2):399-408.

Knott, K., Christian, A., Falcone, J. F., Vance, C., Bauer, L. L., Fahey Jr., G. C., Kouba, A. J. 2017. Phenological changes in bamboo carbohydrates explain the preference for culm over leaves by giant pandas (Ailuropoda melanoleuca) during spring. Plos One 12(6).

Knott, K. K., Mastromonaco, G. F., Owen, M. A., Kouba, A. J. 2017. Urinary profiles of progestin and androgen metabolites in female polar bears during parturient and non-parturient cycles. Conservation Physiology 5(1).

Wang, Y. Z., Dong, W. Y., Kouba, A. J. 2016. Fast discrimination of bamboo species using VIS/NIR spectroscopy. Journal of Applied Spectroscopy 83(5):826-831.

Earl, J. E., Chaney, J. C., Sutton, W. B., Lillard, C. E., Kouba, A. J., Langhorne, C., Krebs, J., Wilkes, R. P., Hill, R. D., Miller, D. L., Gray, M. J. 2016. Ranavirus could facilitate local extinction of rare amphibian species. Oecologia 182(2):611-23.

Graham, K. M., Kouba, A. J., Langhorne, C. J., Marcec, R. M., Willard, S. T. 2016. Biological sex identification in the endangered dusky gopher frog (Lithobates sevosa): a comparison of body size measurements, secondary sex characteristics, ultrasound imaging, and urinary hormone analysis methods. Reproductive Biology and Endocrinology 14(1):41.

Zhang, L., Jiang, W., Wang, Q. J., Zhao, H., Zhang, H. X., Marcec, R. M., Willard, S. T., Kouba, A. J. 2016. Reintroduction and Post-Release Survival of a Living Fossil: The Chinese Giant Salamander. PloS One 11(6):e0156715.

McDonough, C. E., Martin, M. W., Vance, C., Cole, J. A., Kouba, A. J. 2016. Frequency of exogenous hormone therapy impacts spermiation in male Fowler's toad (Bufo fowleri). Reproduction, Fertility, and Development 28(7):995-1003.

Williams, C. L., Dill-McFarland, K. A., Vandewege, M. W., Sparks, D. L., Willard, S. T., Kouba, A. J., Suen, G., Brown, A. E. 2016. Dietary shifts may trigger dysbiosis and mucous stools in giant pandas (Ailuropoda melanoleuca). Frontiers in Microbiology 7.

Marcec, R., Kouba, A. J., Zhang, L., Zhang, H., Wang, Q., Zhao, H., Jiang, W., Willard, S. T. 2016. Surgical implantation of coelomic radiotransmitters and postoperative survival of Chinese Giant Salamanders (Andrias davidianus) following reintroduction. Journal of Zoo and Wildlife Medicine 47(1):187-195.

Christian, A. L., Knott, K. K., Vance, C., Falcone, J. F., Bauer, L. L., Fahey, Jr, G. C., Willard, S. T., Kouba, A. J. 2015. Nutrient and mineral composition during shoot growth in seven species of Phyllostachys and Pseudosasa bamboo consumed by giant panda. Journal of Animal Physiology and Animal Nutrition 99(6):1172-83.

Boyle, S. A., Roberts, B., Pope, B. M., Blake, M. R., Leavelle, S. E., Marshall, J. J., Smith, A., Hadicke, A., Falcone, J. F., Knott, K., Kouba, A. J. 2015. Assessment of Flooring Renovations on African Elephant (Loxodonta africana) Behavior and Glucocorticoid Response. PloS One 10(11):e0141009.

Calatayud, N. E., Langhorne, C. J., Mullen, A. C., Williams, C., Smith, T., Bullock, L., Kouba, A. J., Willard, S. T. 2015. A hormone priming regimen and hibernation affect oviposition in the boreal toad (Anaxyrus boreas boreas). Theriogenology 84(4):600-607.

Vance, C. K., Kouba, A. J., Zhang, H. X., Zhou, H., Wang, Q., Willard, S. T. 2015. Near infrared reflectance spectroscopy studies of Chinese giant salamanders in aquaculture production. NIR News/IM Publications 26(2):4-7.

Sutton, W. B., Gray, M. J., Hardman, R. H., Wilkes, R. P., Kouba, A. J., Miller, D. L. 2014. High susceptibility of the endangered dusky gopher frog to ranavirus. Diseases of Aquatic Organisms 112(1):9-16.

Clulow, J., Trudeau, V. L., Kouba, A. J. 2014. Amphibian declines in the twenty-first century: why we need assisted reproductive technologies. Advances in Experimental Medicine and Biology 753:275-316.

Knott, K. K., Roberts, B. M., Maly, M. A., Vance, C., Debeachaump, J., Majors, J., Riger, P., Decaluwe, H., Kouba, A. J. 2013. Fecal estrogen, progestagen and glucocorticoid metabolites during the estrous cycle and pregnancy in the giant anteater (Myrmecophaga tridactyla): Evidence for delayed implantation. Reproductive Biology and Endocrinology 11:83.

Kouba, A. J., delBarco-Trillo, J., Vance, C., Milam, C., Carr, M. 2012. A comparison of human chorionic gonadotropin and luteinizing hormone releasing hormone on the induction of spermiation and amplexus in the American toad (Anaxyrus americanus). Reproductive Biology and Endocrinology 10:59.

Wiedower, E. E., Kouba, A. J., Vance, C., Hansen, R. L., Stuth, J. W., Tolleson, D. R. 2012. Fecal near infrared spectroscopy to discriminate physiological status in giant pandas. PloS One 7(6):e38908.

Swaisgood, R. R., Wei, F., McShea, W. J., Wildt, D. E., Kouba, A. J., Zhang, Z. 2011. Can science save the giant panda (Ailuropoda melanoleuca)? Unifying science and policy in an adaptive management paradigm. Integrative Zoology 6(3):290-6.

Willis, E. L., Kersey, D. C., Durrant, B. S., Kouba, A. J. 2011. The acute phase protein ceruloplasmin as a non-invasive marker of pseudopregnancy, pregnancy, and pregnancy loss in the giant panda. PloS One 6(7):e21159.

Swaisgood, R. R., Wei, F., Wildt, D. E., Kouba, A. J., Zhang, Z. 2010. Giant panda conservation science: How far we have come. Biology Letters 6(2):143-5.

Hansen, R. L., Carr, M. M., Apanavicius, C. J., Jiang, P., Bissell, H. A., Gocinski, B. L., Maury, F., Himmelreich, M., Beard, S., Ouellette, J. R., Kouba, A. J. 2010. Seasonal shifts in giant panda feeding behavior: Relationships to bamboo plant part consumption. Zoo Biology 29(4):470-483.

Kouba, A. J., Vance, C. 2009. Applied reproductive technologies and genetic resource banking for amphibian conservation. Reproduction, Fertility, and Development 21(6):719-737.

Kouba, A. J., Vance, C., Willis, E. L. 2009. Artificial fertilization for amphibian conservation: Current knowledge and future considerations. Theriogenology 71(1):214-27.

Browne, R. K., Seratt, J., Vance, C., Kouba, A. J. 2006. Hormonal priming, induction of ovulation and in-vitro fertilization of the endangered Wyoming toad (Bufo baxteri). Reproductive Biology and Endocrinology 4:34.

Kouba, A. J., Vance, C., Frommeyer, M. A., Roth, T. L. 2003. Structural and functional aspects of Bufo americanus spermatozoa: Effects of inactivation and reactivation. Journal of Experimental Zoology Part A: Comparative Experimental Biology 295(2):172-182.

Kouba, A. J., Atkinson, M. W., Gandolf, A. R., Roth, T. L. 2001. Species-specific sperm-egg interaction affects the utility of a heterologous bovine in vitro fertilization system for evaluating antelope sperm. Biology of Reproduction 65(4):1246-1251.

Kouba, A. J., Abeydeera, L. R., Alvarez, I. M., Day, B. N., Buhi, W. C. 2000. Effects of the porcine oviduct-specific glycoprotein on fertilization, polyspermy, and embryonic development in vitro. Biology of Reproduction 63(1):242-250.

Kouba, A. J., Burkhardt, B. R., Alvarez, I. M., Goodenow, M. M., Buhi, W. C. 2000. Oviductal plasminogen activator inhibitor-1 (PAI-1): mRNA, protein, and hormonal regulation during the estrous cycle and early pregnancy in the pig. Molecular Reproduction and Development 56(3):378-386.

Kouba, A. J., Alvarez, I. M., Buhi, W. C. 2000. Identification and localization of plasminogen activator inhibitor-1 within the porcine oviduct. Biology of Reproduction 62(3):501-510.

Buhi, W. C., Alvarez, I. M., Kouba, A. J. 2000. Secreted proteins of the oviduct. Cells Tissues Organs 166(2):165-179.

Henricks, D. M., Kouba, A. J., Lackey, B. R., Boone, W. R., Gray, S. L. 1998. Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: Influence on sperm motility. Biology of Reproduction 59(2):330-337.

Buhi, W. C., Alvarez, I. M., Pickard, A. R., McIntush, E. W., Kouba, A. J., Ashworth, C. J., Smith, M. F. 1997. Expression of tissue inhibitor of metalloproteinase-1 protein and messenger ribonucleic acid by the oviduct of cyclic, early-pregnant, and ovariectomized steroid-treated gilts. Biology of Reproduction 57(1):7-15.

Buhi, W. C., Alvarez, I. M., Kouba, A. J. 1997. Oviductal regulation of fertilization and early embryonic development. Journal of Reproduction and Fertility Supplement 52:285-300.